EMD-4463 Experiments and Validation

Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation

Single particle reconstruction
Overview of EMD-4463
Sample name: Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation
Organism: Homo sapiens
Related EM entries by publication: EMD-4464, EMD-4465, EMD-4466, EMD-4467

Map parameters

Recommended contour level: 0.016 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.06 × 1.06 × 1.06 Å
Minimum density: -0.017
Maximum density: 0.067
Average density: 0.00
Standard deviation: 0.003

Sample information

Sample name: Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation
Proteins: Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation, Apc1, Apc2, Apc3, Apc4, Apc5, Apc6, Apc7, Apc8, Apc10, Apc11, Apc12, Apc13, Apc15, Apc16, Cdc20, Cdk2, Cyclin A2, Cks2, BubR1, Mad2, Bub3

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 184058

Imaging

Session
Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 28.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.15
Buffer: 20mM Hepes, 150mM NaCl, 0.5mM TCEP
Vitrification
Cryogen: ETHANE