EMD-4371

Single-particle
9.2 Å
EMD-4371
3D View Gallery
Deposition: 16/04/2018
Map released: 13/06/2018
Last modified: 25/07/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4371
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cryo-EM map of the FCHSD2 F-BAR domain + SH3-1

EMD-4371

Single-particle
9.2 Å
EMD-4371
3D View Gallery
Deposition: 16/04/2018
Map released: 13/06/2018
Last modified: 25/07/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.15 mg/mL
Buffer
pH: 7.5
Buffer components [2]:
Name Formula Concentration ChEBI
Sodium Chloride NaCl 150.0 mM
HEPES C8H18N2O4S 20.0 mM
Grid
Mesh: 300
Model: Quantifoil R1.2/1.3
Material: GOLD
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Number of real images: 766
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 9.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 30270
Algorithm: FOURIER SPACE
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
RELION 2.0 -
Startup model [1]
Type: PDB ENTRY
PDB model: 4DYL
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 2.0 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 2.0 -
Particle selection [1]
Selected Ref. model Method Software Details
230954 - - - -
Final 3D classification
Number of classes: 4
Software [1]
Name Version Details
RELION 2.0 -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: FCHSD2 F-BAR SH3-1 domains.
⬡ Geometry
X Y Z
Dimensions 218 218 218
Origin 0 0 0
Spacing 218 218 218
Voxel size 1.1 Å 1.1 Å 1.1 Å
Contour list
Primary Level Source
True 0.0123 AUTHOR