EMD-4274

Single-particle
4.1 Å
EMD-4274
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Deposition: 26/01/2018
Map released: 07/03/2018
Last modified: 11/12/2019
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4274
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EMICSS annotations (xml)

CryoEM structure of E.coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA

EMD-4274

Single-particle
4.1 Å
EMD-4274
3D View Gallery
Deposition: 26/01/2018
Map released: 07/03/2018
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Cryo-EM structure of E. coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA
Complex [3]
Name: Cryo-EM structure of E. coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA
Complex Portal [7]
ID Name Overlap
CPX-4884 DNA-directed RNA polymerase holoenzyme complex, Sigma54 variant 0.60
CPX-4886 DNA-directed RNA polymerase holoenzyme complex, SigmaF variant 0.60
CPX-4881 DNA-directed RNA polymerase holoenzyme complex, Sigma70 variant 0.60
CPX-4888 DNA-directed RNA polymerase holoenzyme complex, Sigma fecI variant 0.60
CPX-4883 DNA-directed RNA polymerase holoenzyme complex, SigmaS variant 0.60
CPX-4885 DNA-directed RNA polymerase holoenzyme complex, SigmaE variant 0.60
CPX-4887 DNA-directed RNA polymerase holoenzyme complex, SigmaH variant 0.60
Name: RNA polymerase and NusA
Natural source [1]
Organism: Escherichia coli K-12
Recombinant Expression [1]
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
Complex Portal [7]
ID Name Overlap
CPX-4885 DNA-directed RNA polymerase holoenzyme complex, SigmaE variant 0.60
CPX-4886 DNA-directed RNA polymerase holoenzyme complex, SigmaF variant 0.60
CPX-4888 DNA-directed RNA polymerase holoenzyme complex, Sigma fecI variant 0.60
CPX-4883 DNA-directed RNA polymerase holoenzyme complex, SigmaS variant 0.60
CPX-4881 DNA-directed RNA polymerase holoenzyme complex, Sigma70 variant 0.60
CPX-4884 DNA-directed RNA polymerase holoenzyme complex, Sigma54 variant 0.60
CPX-4887 DNA-directed RNA polymerase holoenzyme complex, SigmaH variant 0.60
Name: Nucleic acids
Natural source [1]
Organism: synthetic construct
Recombinant Expression [1]
Organism Strain Cell Plasmid
synthetic construct - - -
Protein [4]
LEVO
Name: DNA-directed RNA polymerase subunit alpha
EC number: 2.7.7.6
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [25]
Biological process:
intracellular iron ion homeostasis DNA-templated transcription initiation regulation of DNA-templated transcription elongation DNA-templated transcription regulation of DNA-templated transcription initiation bacterial-type flagellum assembly cell motility nitrate assimilation response to heat submerged biofilm formation iron ion homeostasis regulation of DNA-templated transcription, elongation cellular response to cell envelope stress bacterial-type flagellum-dependent cell motility regulation of DNA-templated transcription, initiation transcription, DNA-templated transcription antitermination
Molecular function:
DNA-directed 5'-3' RNA polymerase activity protein dimerization activity DNA binding
Cellular location:
transcription elongation factor complex cytosol membrane cytoplasm cytosolic DNA-directed RNA polymerase complex
Natural source
Organism: Escherichia coli (strain K12)
Strain: K12
Molecular weight
Experimental Theoretical Method
- 36 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [21]
Biological process:
bacterial-type flagellum-dependent cell motility cellular response to cell envelope stress response to heat regulation of DNA-templated transcription, elongation cell motility submerged biofilm formation bacterial-type flagellum assembly nitrate assimilation iron ion homeostasis response to antibiotic transcription antitermination transcription, DNA-templated regulation of DNA-templated transcription, initiation
Molecular function:
DNA binding ribonucleoside binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
transcription elongation factor complex cytosolic DNA-directed RNA polymerase complex cytoplasm membrane cytosol
Natural source
Organism: Escherichia coli (strain K12)
Strain: K12
Molecular weight
Experimental Theoretical Method
- 150 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [27]
Biological process:
intracellular iron ion homeostasis DNA-templated transcription initiation DNA-templated transcription regulation of DNA-templated transcription initiation regulation of DNA-templated transcription elongation response to heat submerged biofilm formation nitrate assimilation regulation of DNA-templated transcription, initiation response to antibiotic bacterial-type flagellum assembly iron ion homeostasis transcription, DNA-templated bacterial-type flagellum-dependent cell motility regulation of DNA-templated transcription, elongation transcription antitermination cellular response to cell envelope stress cell motility
Molecular function:
magnesium ion binding zinc ion binding DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
membrane cytosol cytosolic DNA-directed RNA polymerase complex transcription elongation factor complex cytoplasm
Natural source
Organism: Escherichia coli (strain K12)
Strain: K12
Molecular weight
Experimental Theoretical Method
- 155 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: DNA-directed RNA polymerase subunit omega
EC number: 2.7.7.6
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [24]
Biological process:
intracellular iron ion homeostasis DNA-templated transcription initiation DNA-templated transcription regulation of DNA-templated transcription elongation regulation of DNA-templated transcription initiation bacterial-type flagellum-dependent cell motility cellular response to cell envelope stress regulation of DNA-templated transcription, elongation response to heat bacterial-type flagellum assembly nitrate assimilation iron ion homeostasis submerged biofilm formation cell motility transcription, DNA-templated regulation of DNA-templated transcription, initiation protein-containing complex assembly transcription antitermination
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
transcription elongation factor complex cytosolic DNA-directed RNA polymerase complex cytosol RNA polymerase complex
Natural source
Organism: Escherichia coli (strain K12)
Strain: K12
Molecular weight
Experimental Theoretical Method
- 10 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
DNA [2]
DNA
Name: DNA (30-MER)
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 9 kDa -
DNA
Name: DNA (39-MER)
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 11 kDa -
RNA [1]
Name: RNA (5'-R(P*GP*AP*UP*GP*UP*GP*UP*GP*CP*U)-3')
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 3 kDa -
Ligand [2]
Name: MAGNESIUM ION
HET code: MG
Number of copies: 1
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -
Name: ZINC ION
HET code: ZN
Number of copies: 2
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 65 Da -