EMD-4253

Single-particle
6.1 Å
EMD-4253
3D View Gallery
Deposition: 03/01/2018
Map released: 07/02/2018
Last modified: 07/02/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4253
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Human Bact spliceosome state 7 core

EMD-4253

Single-particle
6.1 Å
EMD-4253
3D View Gallery
Deposition: 03/01/2018
Map released: 07/02/2018
Last modified: 07/02/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.05 mg/mL
Buffer
pH: 7.9
Buffer components [3]:
Name Formula Concentration ChEBI
HEPES - 20.0 mM -
- NaCl 150.0 mM
- MgCl2 1.5 mM
Grid
Mesh: 400
Model: Quantifoil R3.5/1
Material: COPPER
Support Film [2]
Material Topology Thickness
CARBON HOLEY -
CARBON CONTINUOUS -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 75%
Chamber temperature: 277 K
Instrument: LEICA EM GP
Details: Blot with blotting sensor.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 0.001 mm
Nominal defocus: 0.8 µm - 4.5 µm
Nominal magnification: 59000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE ( Residual tilt: 14.0 mrad)
Specialist optics
SPH aberration_corrector: Microscope was modified with a Cs corrector with two hexapoles elements
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Dimensions: 4096 pixel x 4096 pixel
Sampling interval: 14.0 µm
Number of grids: 1
Number of real images: 32000
Average exposure time: 1.0 s
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 6.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 30793
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 2.1 -
⦨ Initial angle assignment
Type: OTHER
Software [1]
Name Version Details
EMAN2 - -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION - -
Particle selection [1]
Selected Ref. model Method Software Details
3300000 - - - -
Final 3D classification
Number of classes: 220
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 326 326 326
Origin 0 0 0
Spacing 326 326 326
Voxel size 1.5 Å 1.5 Å 1.5 Å
Contour list
Primary Level Source
True 0.0421 AUTHOR