EMD-4233 Experiments and Validation

Human Bact spliceosome state 1 unmasked

Single particle reconstruction
Overview of EMD-4233
Sample name: human Bact spliceosome state 1 unmasked
Organism: Homo sapiens

Map parameters

Recommended contour level: 0.008 (author)
Number of grid points: 420 × 420 × 420
Voxel size: 1.16 × 1.16 × 1.16 Å
Minimum density: -0.016
Maximum density: 0.037
Average density: 0.001
Standard deviation: 0.004

Sample information

Sample name: human Bact spliceosome state 1 unmasked
Protein: human Bact spliceosome state 1 unmasked

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 17000

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 40.0
Nominal CS (mm): 0.001
Nominal magnification: 59000.0
Defocus max (nm): 4,500.00
Defocus min (nm): 800.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Acquisition
Number of images: 32000
Sampling interval (μm): 14.0

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.05
Vitrification
Apparatus: LEICA EM GP
Cryogen: ETHANE
Humidity: 75.0
Details: blot with blotting sensor