EMD-4155 Experiments and Validation

Ribosome-bound membrane insertase YidC

Single particle reconstruction
Overview of EMD-4155
Sample name: Complex of translationally-stalled ribosome and membrane-embedded YidC insertase
Organism: Escherichia coli
Fitted atomic model: 5m5h

Map parameters

Recommended contour level: 0.015 (author)
Number of grid points: 450 × 450 × 450
Voxel size: 1.08 × 1.08 × 1.08 Å
Minimum density: -0.04
Maximum density: 0.091
Average density: 0.001
Standard deviation: 0.005

Sample information

Sample name: Complex of translationally-stalled ribosome and membrane-embedded YidC insertase
Protein: Complex of translationally-stalled ribosome and membrane-embedded YidC insertase

Validation information

Experimental information

 

Image processing

Number of particles: 42658

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 24.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Buffer: 150 mM KOAc, 5 mM Mg(OAc)2, 25 mM HEPES pH 7.2
Vitrification
Cryogen: ETHANE