EMD-4071 Experiments and Validation

Structure of the 40S ABCE1 post-splitting complex in ribosome recycling and translation initiation

Single particle reconstruction
Overview of EMD-4071
Sample name: 40S-ABCE1 complex
Organism: Saccharomyces cerevisiae
Fitted atomic model: 5ll6
Related EM entry by publication: EMD-3452

Map parameters

Recommended contour level: 0.038 (emdb)
Number of grid points: 384 × 384 × 384
Voxel size: 1.08 × 1.08 × 1.08 Å
Minimum density: -0.195
Maximum density: 0.472
Average density: 0.00
Standard deviation: 0.013

Sample information

Sample name: 40S-ABCE1 complex
Protein: 40S-ABCE1 complex

Validation information

Experimental information

 

Image processing

Software: RELION
Number of particles: 102000

Fitting

Fitting protocol: flexible
Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 2.3999999999999999
Nominal CS (mm): 2.70
Defocus max (nm): 2,400.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100