EMD-4038 Function and Biology

Cryo-EM structure of RecBCD+DNA complex revealing activated nuclease domain

Single particle reconstruction
3.83Å resolution
Overview of EMD-4038
Sample name: Ternary complex of RecBCD with forked DNA substrate and ADPNP
Number of unique components: 7

Ternary complex of RecBCD with forked DNA substrate and ADPNP [Escherichia coli K12]

Protein type
Name: Ternary complex of RecBCD with forked DNA substrate and ADPNP
Theoretical weight (MDa): 0.35
Expression system
Expression system: Escherichia coli
Strain: B834
Vector: 3 plasmids

RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB [Escherichia coli (strain K12)]

Protein type
Name: RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB
Theoretical weight (MDa): 0.134
Number of copies: 1
Expression system
Expression system: Escherichia coli

RecBCD enzyme subunit RecC [Escherichia coli (strain K12)]

Protein type
Name: RecBCD enzyme subunit RecC
Theoretical weight (MDa): 0.129
Number of copies: 1
Expression system
Expression system: Escherichia coli

RecBCD enzyme subunit RecD [Escherichia coli (strain K12)]

Protein type
Name: RecBCD enzyme subunit RecD
Theoretical weight (MDa): 0.067
Number of copies: 1
Expression system
Expression system: Escherichia coli

Fork-Hairpin DNA (70-MER) [Endothia gyrosa]

Nucleic-acid type
Name: Fork-Hairpin DNA (70-MER)
Theoretical weight (MDa): 0.021
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DA)(DA)(DT)(DG)(DC) (DG)(DA)(DG)(DC)(DA)(DC)(DT)(DG)(DC)(DT)(DA)(DC)(DA)(DG)(DC)(DA)(DT)(DT)(DT)(DC) (DC)(DC)(DA)(DT)(DG)(DC)(DT)(DG)(DT)(DA)(DG)(DC)(DA)(DG)(DT)(DG)(DC)(DT)(DC)(DG) (DC)(DA)(DT)(DT)(DA)(DG)(DA)(DT)(DT)(DT)

PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

Ligand type
Name: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
Theoretical weight (MDa): 0.001
Number of copies: 1

MAGNESIUM ION

Ligand type
Name: MAGNESIUM ION
Theoretical weight (MDa): 0
Number of copies: 1