EMD-3998 Experiments and Validation

N0 and N1 ring of PilQ from Thermus thermophilus

Single particle reconstruction
Overview of EMD-3998
Sample name: PilQ complex with 13-fold symmetry
Organism: Thermus thermophilus HB27
Related EM entries by publication: EMD-3985, EMD-3995, EMD-3996, EMD-3997

Map parameters

Recommended contour level: 0.034 (author)
Number of grid points: 324 × 324 × 324
Voxel size: 1.63 × 1.63 × 1.63 Å
Minimum density: -0.06
Maximum density: 0.126
Average density: 0.00
Standard deviation: 0.003

Sample information

Sample name: PilQ complex with 13-fold symmetry
Protein: PilQ complex with 13-fold symmetry

Validation information

Experimental information

 

Image processing

Applied symmetry: C13
Software: FREALIGN
Number of particles: 2646

Imaging

Session
Microscope model: JEOL 3200FSC
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 33.0
Energy filter: In-column Omega Filter
Energy window (eV): 18.00
Nominal CS (mm): 4.20
Nominal magnification: 20000.0
Holder model: JEOL

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 70.0
Details: blotting for 8-10 seconds