EMD-3985
PilQ from Thermus thermophilus
EMD-3985
Single-particle20.4 Å
Deposition: 15/11/2017
Map released: 10/01/2018
Last modified: 28/03/2018
Buffer
pH: 8.5
Grid
Mesh: 400
Model: Quantifoil R1.2/1.3
Material: COPPER
Model: Quantifoil R1.2/1.3
Material: COPPER
Pretreatment
Support Film [1]
Material | Topology | Thickness |
---|---|---|
CARBON | CONTINUOUS | - |
Vitrification
Cryogen name: ETHANE
Chamber humidity: 70%
Chamber temperature: 283 K
Instrument: FEI VITROBOT MARK IV
Details: Blotting for 8-10 seconds.
Chamber humidity: 70%
Chamber temperature: 283 K
Instrument: FEI VITROBOT MARK IV
Details: Blotting for 8-10 seconds.
Microscope: JEOL 3200FSC
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 4.2 mm
Nominal magnification: 20000.0
Calibrated magnification: 30675.0
Specimen holder model: JEOL
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 4.2 mm
Nominal magnification: 20000.0
Calibrated magnification: 30675.0
Specimen holder model: JEOL
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Specialist optics
Energy filter
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Frames per image: 2-45
Average exposure time: 9.0 s
Average electron dose per image: 33.0 e/Å2
Detector mode: COUNTING
Frames per image: 2-45
Average exposure time: 9.0 s
Average electron dose per image: 33.0 e/Å2
Final
reconstruction
Resolution: 20.4
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 2646
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 2646
⌯ Applied Symmetry
Point group:
C13
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.4 | - |
Startup model
[1]
Particle selection
[1]
Selected | Ref. model | Method | Software | Details |
---|---|---|---|---|
11457 | - | - | - | - |
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 4 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 324 | 324 | 324 |
Origin | 0 | 0 | 0 |
Spacing | 324 | 324 | 324 |
Voxel size | 1.63 Å | 1.63 Å | 1.63 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.025 | AUTHOR |