EMD-3821

Single-particle
7.4 Å
EMD-3821
3D View Gallery
Deposition: 21/07/2017
Map released: 07/02/2018
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3821
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6eqc summary report (pdf.gz) Map and 6eqc FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM reconstruction of a modified human Adenovirus C5

EMD-3821

Single-particle
7.4 Å
EMD-3821
3D View Gallery
Deposition: 21/07/2017
Map released: 07/02/2018
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average exposure time: 0.4 s
Average electron dose per image: 1.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 7.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 1880
Software [1]
Name Version Details
RELION 1.4 -
Startup model [1]
Type: PDB ENTRY
PDB model: 4CWU
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
CTF correction
Software [1]
Name Version Details
CTFFIND 4.0.17 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 800 800 800
Origin 0 0 0
Spacing 800 800 800
Voxel size 1.96 Å 1.96 Å 1.96 Å
Contour list
Primary Level Source
True 0.014 AUTHOR