EMD-3748

Single-particle
3.45 Å
EMD-3748 Deposition: 02/06/2017
Map released: 12/07/2017
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3748

Cryo-EM reconstruction of the small subunit of the Mycobacterium smegmatis ribosome

EMD-3748

Single-particle
3.45 Å
EMD-3748 Deposition: 02/06/2017
Map released: 12/07/2017
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Model: Quantifoil R2/2
Material: COPPER
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 96%
Instrument: FEI VITROBOT MARK I
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Calibrated magnification: 100719.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: INTEGRATING
Average electron dose per image: 20.0 e/Å2
Details: FEI EPU data collection
Image processing [1]
Final reconstruction
Resolution: 3.45 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 224584
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 1.4 -
Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 1.4 -
Final angle assignment
Type: OTHER
Software [1]
Name Version Details
RELION 1.4 -
CTF correction
Details:CTF correction in Relion
Software [1]
Name Version Details
CTFFIND 3 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Geometry
X Y Z
Dimensions 216 216 216
Origin 0 0 0
Spacing 216 216 216
Voxel size 1.39 Å 1.39 Å 1.39 Å
Contour list
Primary Level Source
True 0.1 AUTHOR