EMD-3748
Cryo-EM reconstruction of the small subunit of the Mycobacterium smegmatis ribosome
EMD-3748
Single-particle3.45 Å
Deposition: 02/06/2017
Map released: 12/07/2017
Last modified: 11/12/2019
Buffer
pH: 7.5
Grid
Vitrification
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Calibrated magnification: 100719.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Calibrated magnification: 100719.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
FEI FALCON II (4k x 4k)
Detector mode: INTEGRATING
Average electron dose per image: 20.0 e/Å2
Details: FEI EPU data collection
Detector mode: INTEGRATING
Average electron dose per image: 20.0 e/Å2
Details: FEI EPU data collection
Final
reconstruction
Resolution: 3.45
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 224584
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 224584
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.4 | - |
⦨ Initial angle
assignment
CTF correction
Details:CTF correction in Relion
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 3 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 216 | 216 | 216 |
Origin | 0 | 0 | 0 |
Spacing | 216 | 216 | 216 |
Voxel size | 1.39 Å | 1.39 Å | 1.39 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.1 | AUTHOR |