EMD-3534 Experiments and Validation

26S proteasome in presence of ATP (s1)

Single particle reconstruction
Overview of EMD-3534
Sample name: 26S proteasome of Saccharomyces cerevisiae in presence of ATP (s1)
Organisms: Saccharomyces cerevisiae S288c, Baker's yeast
Fitted atomic models: 5mp9, 5mpd
Related EM entries by publication: EMD-3535, EMD-3536, EMD-3537

Map parameters

Recommended contour level: 0.02 (author)
Number of grid points: 384 × 384 × 384
Voxel size: 1.38 × 1.38 × 1.38 Å
Minimum density: -0.133
Maximum density: 0.211
Average density: -0.00
Standard deviation: 0.006

Sample information

Sample name: 26S proteasome of Saccharomyces cerevisiae in presence of ATP (s1)
Ligands: ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE
Proteins: 26S proteasome of Saccharomyces cerevisiae in presence of ATP (s1), Proteasome subunit alpha type-1, Proteasome subunit alpha type-2, Proteasome subunit alpha type-3, Proteasome subunit alpha type-4, Proteasome subunit alpha type-5, Proteasome subunit alpha type-6, Probable proteasome subunit alpha type-7, Proteasome subunit beta type-1, Proteasome subunit beta type-2, Proteasome subunit beta type-3, Proteasome subunit beta type-4, Proteasome subunit beta type-5, Proteasome subunit beta type-6, Proteasome subunit beta type-7, 26S protease regulatory subunit 7 homolog, 26S protease regulatory subunit 4 homolog, 26S protease regulatory subunit 6B homolog, 26S protease subunit RPT4, 26S protease regulatory subunit 6A, 26S protease regulatory subunit 8 homolog

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 286500

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 45.0
Defocus max (nm): 3,500.00
Defocus min (nm): 1,500.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: OTHER
Humidity: 95