EMD-3297

Single-particle
3.3 Å
EMD-3297
3D View Gallery
Deposition: 12/01/2016
Map released: 27/01/2016
Last modified: 09/03/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3297
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5ftl summary report (pdf.gz) Map and 5ftl FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of human p97 bound to ATPgS (Conformation I)

EMD-3297

Single-particle
3.3 Å
EMD-3297
3D View Gallery
Deposition: 12/01/2016
Map released: 27/01/2016
Last modified: 09/03/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.9 mg/mL
Buffer
pH: 8.0
Details: 25 mM Tris, 150 mM NaCl, 1 mM MgCl2, 1.0 mM TCEP
Grid
Details: 200 mesh Quantifoil R1.2/1/3 grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 90.15 K
Instrument: FEI VITROBOT MARK IV
Method: Blot for 2.5 seconds before plunging.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.95 µm - 2.7 µm
Nominal magnification: 105000.0
Calibrated magnification: 36980.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Specimen holder details: Liquid nitrogen cooled
Details: Parallel beam illumination
Temperature
Minimum: 79.6 K
Average: 79.7 K
Maximum: 79.8 K
Specialist optics
Energy filter
Name: Gatan, Inc.
Lower energy threshold: 0.0 eV
Upper energy threshold: 20.0 eV
Image Recording [1]
Detector category: CCD
Detector model: GATAN K2 SUMMIT (4k x 4k)
Number of real images: 628
Average electron dose per image: 40 e/Å2
Details: Every image is the average of 40 frames recorded by the direct electron detector.
Image processing [1]
Final reconstruction
Resolution: 3.3 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 33882
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C6
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of human p97 bound to ATPgS (conformation I).
Details: ::::EMDATABANK.org::::EMD-3297::::
⬡ Geometry
X Y Z
Dimensions 276 276 276
Origin 0 0 0
Spacing 276 276 276
Voxel size 0.676 Å 0.676 Å 0.676 Å
Contour list
Primary Level Source
True 0.0179 AUTHOR