EMD-3146

Single-particle
4.1 Å
EMD-3146
3D View Gallery
Deposition: 06/09/2015
Map released: 21/10/2015
Last modified: 02/12/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3146
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5anb summary report (pdf.gz) Map and 5anb FULL report (pdf.gz)
EMICSS annotations (xml)

Mechanism of eIF6 release from the nascent 60S ribosomal subunit

EMD-3146

Single-particle
4.1 Å
EMD-3146
3D View Gallery
Deposition: 06/09/2015
Map released: 21/10/2015
Last modified: 02/12/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Dictyostelium 60S carrying endogenous eIF6 with recombinant human SBDS and human EFL1
Sample [1]
Name: Dictyostelium 60S carrying endogenous eIF6 with recombinant human SBDS and human EFL1
Number of unique components: 4
Complex [1]
Name: 60S ribosomal subunit
Ribosome details: Ribosome-eukaryote: LSU 60S
Natural source [1]
Organism: Dictyostelium discoideum (Slime Mold)
Strain: HM2917
Recombinant Expression [1]
Organism Strain Cell Plasmid
- - - -
Molecular weight
Experimental Theoretical Method
3 MDa 3 MDa -
Protein [3]
Name: Eukaryotic translation initiation factor 6 (eIF6)
Number of copies: 1
Details: Endogenous dictyostelium protein purified bound the 60S ribosomal subunit
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Dictyostelium discoideum (Slime Mold)
Strain: HM2917
Molecular weight
Experimental Theoretical Method
26 kDa 26 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Recombinant expression
Name: Shwachman-Bodian-Diamond syndrome protein (SBDS)
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Homo sapiens (Human)
Molecular weight
Experimental Theoretical Method
29 kDa 29 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) C41 - pRSETA
Recombinant expression
Name: Elongation factor-like 1 (EFL1)
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Homo sapiens (Human)
Molecular weight
Experimental Theoretical Method
125 kDa 125 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae BCY123 - pYES2