EMD-30577
cryo-EM structure of human RNA polymerase III in elongating state
EMD-30577
Single-particle2.9 Å
Deposition: 25/09/2020
Map released: 17/02/2021
Last modified: 27/03/2024
Name: Human Pol3 EC
Summary:
- Complex
- Protein
- DNA-directed RNA polymerase III subunit RPC1
- DNA-directed RNA polymerase III subunit RPC2
- DNA-directed RNA polymerases I and III subunit RPAC1
- DNA-directed RNA polymerase III subunit RPC9
- DNA-directed RNA polymerases I, II, and III subunit RPABC1
- DNA-directed RNA polymerases I, II, and III subunit RPABC2
- DNA-directed RNA polymerase III subunit RPC8
- DNA-directed RNA polymerases I, II, and III subunit RPABC3
- DNA-directed RNA polymerase III subunit RPC10
- DNA-directed RNA polymerases I, II, and III subunit RPABC5
- DNA-directed RNA polymerases I and III subunit RPAC2
- DNA-directed RNA polymerases I, II, and III subunit RPABC4
- DNA-directed RNA polymerase III subunit RPC5
- DNA-directed RNA polymerase III subunit RPC4
- DNA-directed RNA polymerase III subunit RPC3
- DNA-directed RNA polymerase III subunit RPC6
- DNA-directed RNA polymerase III subunit RPC7
- DNA
- RNA
- Ligand
Human Pol3 EC
Name: Natural source [1]
Organism: Homo sapiens
Complex Portal [1]
ID | Name | Overlap |
---|---|---|
CPX-2393 | DNA-directed RNA polymerase III complex, POLR3G variant | 1.00 |
DNA-directed RNA polymerase III subunit RPC1
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [20]
Biological process:
tRNA transcription by RNA polymerase III transcription by RNA polymerase III DNA-templated transcription positive regulation of interferon-beta production defense response to virus innate immune response transcription, DNA-templated
Molecular function:
zinc ion binding magnesium ion binding DNA/RNA hybrid binding chromatin binding DNA-directed 5'-3' RNA polymerase activity DNA binding metal ion binding
Cellular location:
cytoplasm nucleus membrane cytosol nucleoplasm RNA polymerase III complex
tRNA transcription by RNA polymerase III transcription by RNA polymerase III DNA-templated transcription positive regulation of interferon-beta production defense response to virus innate immune response transcription, DNA-templated
Molecular function:
zinc ion binding magnesium ion binding DNA/RNA hybrid binding chromatin binding DNA-directed 5'-3' RNA polymerase activity DNA binding metal ion binding
Cellular location:
cytoplasm nucleus membrane cytosol nucleoplasm RNA polymerase III complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 155 kDa | - |
DNA-directed RNA polymerase III subunit RPC2
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [18]
Biological process:
snRNA transcription by RNA polymerase III transcription by RNA polymerase III innate immune response positive regulation of innate immune response positive regulation of interferon-beta production transcription, DNA-templated defense response to virus
Molecular function:
zinc ion binding DNA/RNA hybrid binding DNA binding metal ion binding ribonucleoside binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
nucleus cytoplasm cytosol nucleoplasm RNA polymerase III complex
snRNA transcription by RNA polymerase III transcription by RNA polymerase III innate immune response positive regulation of innate immune response positive regulation of interferon-beta production transcription, DNA-templated defense response to virus
Molecular function:
zinc ion binding DNA/RNA hybrid binding DNA binding metal ion binding ribonucleoside binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
nucleus cytoplasm cytosol nucleoplasm RNA polymerase III complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 127 kDa | - |
DNA-directed RNA polymerases I and III subunit RPAC1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
transcription by RNA polymerase I
Molecular function:
RNA polymerase I activity protein dimerization activity DNA-directed 5'-3' RNA polymerase activity RNA polymerase III activity DNA binding
Cellular location:
nucleus cytoplasm fibrillar center nucleoplasm RNA polymerase III complex RNA polymerase I complex cytosol
transcription by RNA polymerase I
Molecular function:
RNA polymerase I activity protein dimerization activity DNA-directed 5'-3' RNA polymerase activity RNA polymerase III activity DNA binding
Cellular location:
nucleus cytoplasm fibrillar center nucleoplasm RNA polymerase III complex RNA polymerase I complex cytosol
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 39 kDa | - |
DNA-directed RNA polymerase III subunit RPC9
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
transcription initiation at RNA polymerase III promoter transcription initiation from RNA polymerase III promoter innate immune response defense response to virus transcription by RNA polymerase III neuropeptide signaling pathway
Molecular function:
nucleotide binding DNA-directed 5'-3' RNA polymerase activity calcitonin gene-related peptide receptor activity
Cellular location:
plasma membrane DNA polymerase III complex cytosol nucleoplasm RNA polymerase III complex acrosomal vesicle
transcription initiation at RNA polymerase III promoter transcription initiation from RNA polymerase III promoter innate immune response defense response to virus transcription by RNA polymerase III neuropeptide signaling pathway
Molecular function:
nucleotide binding DNA-directed 5'-3' RNA polymerase activity calcitonin gene-related peptide receptor activity
Cellular location:
plasma membrane DNA polymerase III complex cytosol nucleoplasm RNA polymerase III complex acrosomal vesicle
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 16 kDa | - |
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
transcription by RNA polymerase I transcription by RNA polymerase III protein stabilization transcription by RNA polymerase II
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
RNA polymerase II, core complex nucleoplasm cytosol nucleus RNA polymerase III complex RNA polymerase I complex RPAP3/R2TP/prefoldin-like complex
transcription by RNA polymerase I transcription by RNA polymerase III protein stabilization transcription by RNA polymerase II
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
RNA polymerase II, core complex nucleoplasm cytosol nucleus RNA polymerase III complex RNA polymerase I complex RPAP3/R2TP/prefoldin-like complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 24 kDa | - |
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
tRNA transcription by RNA polymerase III transcription by RNA polymerase I transcription by RNA polymerase II
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
fibrillar center RNA polymerase III complex RNA polymerase I complex nucleus cytosol RNA polymerase II, core complex nucleoplasm
tRNA transcription by RNA polymerase III transcription by RNA polymerase I transcription by RNA polymerase II
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
fibrillar center RNA polymerase III complex RNA polymerase I complex nucleus cytosol RNA polymerase II, core complex nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 14 kDa | - |
DNA-directed RNA polymerase III subunit RPC8
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
transcription initiation at RNA polymerase III promoter transcription initiation from RNA polymerase III promoter nucleobase-containing compound metabolic process transcription by RNA polymerase III defense response to virus innate immune response
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
cytosol RNA polymerase III complex intracellular membrane-bounded organelle nucleoplasm centrosome
transcription initiation at RNA polymerase III promoter transcription initiation from RNA polymerase III promoter nucleobase-containing compound metabolic process transcription by RNA polymerase III defense response to virus innate immune response
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
cytosol RNA polymerase III complex intracellular membrane-bounded organelle nucleoplasm centrosome
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 22 kDa | - |
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
transcription by RNA polymerase I transcription by RNA polymerase III transcription by RNA polymerase II
Molecular function:
DNA-directed 5'-3' RNA polymerase activity single-stranded DNA binding
Cellular location:
nucleus RNA polymerase III complex RNA polymerase II, core complex nucleoplasm protein-DNA complex cytosol RNA polymerase I complex
transcription by RNA polymerase I transcription by RNA polymerase III transcription by RNA polymerase II
Molecular function:
DNA-directed 5'-3' RNA polymerase activity single-stranded DNA binding
Cellular location:
nucleus RNA polymerase III complex RNA polymerase II, core complex nucleoplasm protein-DNA complex cytosol RNA polymerase I complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 17 kDa | - |
DNA-directed RNA polymerase III subunit RPC10
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
termination of RNA polymerase III transcription tRNA 3'-trailer cleavage transcription by RNA polymerase III innate immune response defense response to virus
Molecular function:
DNA-directed 5'-3' RNA polymerase activity zinc ion binding nucleic acid binding
Cellular location:
cytosol RNA polymerase III complex nucleolus nucleoplasm
termination of RNA polymerase III transcription tRNA 3'-trailer cleavage transcription by RNA polymerase III innate immune response defense response to virus
Molecular function:
DNA-directed 5'-3' RNA polymerase activity zinc ion binding nucleic acid binding
Cellular location:
cytosol RNA polymerase III complex nucleolus nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 12 kDa | - |
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
regulation of transcription by RNA polymerase I transcription by RNA polymerase II transcription by RNA polymerase I tRNA transcription by RNA polymerase III
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity zinc ion binding
Cellular location:
RNA polymerase III complex nucleoplasm RNA polymerase II, core complex cytosol nucleus RNA polymerase I complex
regulation of transcription by RNA polymerase I transcription by RNA polymerase II transcription by RNA polymerase I tRNA transcription by RNA polymerase III
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity zinc ion binding
Cellular location:
RNA polymerase III complex nucleoplasm RNA polymerase II, core complex cytosol nucleus RNA polymerase I complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 7 kDa | - |
DNA-directed RNA polymerases I and III subunit RPAC2
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [8]
Biological process:
transcription by RNA polymerase III transcription by RNA polymerase I transcription, DNA-templated
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity protein dimerization activity
Cellular location:
RNA polymerase III complex RNA polymerase I complex
transcription by RNA polymerase III transcription by RNA polymerase I transcription, DNA-templated
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity protein dimerization activity
Cellular location:
RNA polymerase III complex RNA polymerase I complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 15 kDa | - |
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
transcription by RNA polymerase I transcription by RNA polymerase III regulation of transcription by RNA polymerase I transcription by RNA polymerase II
Molecular function:
zinc ion binding DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
RNA polymerase III complex nucleoplasm RNA polymerase II, core complex cytosol nucleus RNA polymerase I complex
transcription by RNA polymerase I transcription by RNA polymerase III regulation of transcription by RNA polymerase I transcription by RNA polymerase II
Molecular function:
zinc ion binding DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
RNA polymerase III complex nucleoplasm RNA polymerase II, core complex cytosol nucleus RNA polymerase I complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 7 kDa | - |
DNA-directed RNA polymerase III subunit RPC5
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [8]
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 80 kDa | - |
DNA-directed RNA polymerase III subunit RPC4
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
tRNA transcription by RNA polymerase III defense response to virus innate immune response positive regulation of innate immune response transcription by RNA polymerase III positive regulation of interferon-beta production
Molecular function:
chromatin binding DNA binding
Cellular location:
RNA polymerase III complex cytosol nucleoplasm
tRNA transcription by RNA polymerase III defense response to virus innate immune response positive regulation of innate immune response transcription by RNA polymerase III positive regulation of interferon-beta production
Molecular function:
chromatin binding DNA binding
Cellular location:
RNA polymerase III complex cytosol nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 44 kDa | - |
DNA-directed RNA polymerase III subunit RPC3
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
DNA-templated transcription transcription by RNA polymerase III positive regulation of interferon-beta production innate immune response transcription, DNA-templated positive regulation of innate immune response regulation of transcription by RNA polymerase III defense response to virus
Molecular function:
single-stranded DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
cytosol RNA polymerase III complex nucleoplasm
DNA-templated transcription transcription by RNA polymerase III positive regulation of interferon-beta production innate immune response transcription, DNA-templated positive regulation of innate immune response regulation of transcription by RNA polymerase III defense response to virus
Molecular function:
single-stranded DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
cytosol RNA polymerase III complex nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 60 kDa | - |
DNA-directed RNA polymerase III subunit RPC6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
positive regulation of interferon-beta production innate immune response defense response to virus positive regulation of innate immune response transcription by RNA polymerase III regulation of transcription by RNA polymerase III
Molecular function:
4 iron, 4 sulfur cluster binding DNA-directed 5'-3' RNA polymerase activity double-stranded DNA binding
Cellular location:
nucleoplasm cytosol RNA polymerase III complex
positive regulation of interferon-beta production innate immune response defense response to virus positive regulation of innate immune response transcription by RNA polymerase III regulation of transcription by RNA polymerase III
Molecular function:
4 iron, 4 sulfur cluster binding DNA-directed 5'-3' RNA polymerase activity double-stranded DNA binding
Cellular location:
nucleoplasm cytosol RNA polymerase III complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 35 kDa | - |
DNA-directed RNA polymerase III subunit RPC7
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
regulation of transcription by RNA polymerase III positive regulation of interferon-beta production positive regulation of innate immune response transcription by RNA polymerase III innate immune response cell population proliferation defense response to virus
Molecular function:
chromatin binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
RNA polymerase III complex nucleus cytosol nuclear body nucleoplasm
regulation of transcription by RNA polymerase III positive regulation of interferon-beta production positive regulation of innate immune response transcription by RNA polymerase III innate immune response cell population proliferation defense response to virus
Molecular function:
chromatin binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
RNA polymerase III complex nucleus cytosol nuclear body nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 25 kDa | - |
Non-template DNA
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 12 kDa | - |
Template DNA
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 12 kDa | - |
RNA
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 6 kDa | - |