EMD-30460

Single-particle
4.0 Å
EMD-30460
3D View Gallery
Deposition: 18/08/2020
Map released: 18/11/2020
Last modified: 10/02/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-30460
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7ct5 summary report (pdf.gz) Map and 7ct5 FULL report (pdf.gz)
EMICSS annotations (xml)

cryo EM map of the S protein of SARS-CoV-2 in complex bound with T-ACE2

EMD-30460

Single-particle
4.0 Å
EMD-30460
3D View Gallery
Deposition: 18/08/2020
Map released: 18/11/2020
Last modified: 10/02/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: GATAN K3 BIOQUANTUM (6k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 57404
Software [1]
Name Version Details
RELION 3.0.6 -
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo EM map of the S protein of SARS-CoV-2 in complex bound with T-ACE2
⬡ Geometry
X Y Z
Dimensions 288 288 288
Origin 0 0 0
Spacing 288 288 288
Voxel size 1.087 Å 1.087 Å 1.087 Å
Contour list
Primary Level Source
True 0.015 AUTHOR