EMD-30261

Single-particle
4.6 Å
EMD-30261
3D View Gallery
Deposition: 28/04/2020
Map released: 28/04/2021
Last modified: 13/10/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-30261
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7bzw summary report (pdf.gz) Map and 7bzw FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of Hsp21

EMD-30261

Single-particle
4.6 Å
EMD-30261
3D View Gallery
Deposition: 28/04/2020
Map released: 28/04/2021
Last modified: 13/10/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 45.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 104126
⌯ Applied Symmetry
Point group: T
Startup model [1]
Type: OTHER
Details:Ab initio from RELION 3.0
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 240 240 240
Origin 0 0 0
Spacing 240 240 240
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.0175 AUTHOR