EMD-30118

Single-particle
4.1 Å
EMD-30118
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Deposition: 14/03/2020
Map released: 14/10/2020
Last modified: 27/03/2024
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-30118
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EMICSS annotations (xml)

CryoEM structure of Thermus thermophilus transcription-repair coupling complex in the presence of ATP-gamma-S

EMD-30118

Single-particle
4.1 Å
EMD-30118
3D View Gallery
Deposition: 14/03/2020
Map released: 14/10/2020
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Thermus thermophilus transcription-repair coupling complex
Complex [1]
Name: Thermus thermophilus transcription-repair coupling complex
Natural source [1]
Organism: Thermus thermophilus
Protein [5]
LEVO
Name: DNA-directed RNA polymerase subunit alpha
EC number: 2.7.7.6
Number of copies: 2
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
protein dimerization activity DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
DNA-directed RNA polymerase complex cytoplasm
Natural source
Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weight
Experimental Theoretical Method
- 35 kDa -
LEVO
Name: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [6]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding ribonucleoside binding
Cellular location:
DNA-directed RNA polymerase complex
Natural source
Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weight
Experimental Theoretical Method
- 125 kDa -
LEVO
Name: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
zinc ion binding magnesium ion binding DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
DNA-directed RNA polymerase complex
Natural source
Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weight
Experimental Theoretical Method
- 170 kDa -
LEVO
Name: DNA-directed RNA polymerase subunit omega
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity
Cellular location:
DNA-directed RNA polymerase complex
Natural source
Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weight
Experimental Theoretical Method
- 11 kDa -
LEVO
Name: Transcription-repair-coupling factor
EC number: 3.6.4.-
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [8]
Biological process:
regulation of DNA-templated transcription regulation of transcription, DNA-templated transcription-coupled nucleotide-excision repair, DNA damage recognition
Molecular function:
damaged DNA binding helicase activity hydrolase activity ATP binding
Cellular location:
cytoplasm
Natural source
Organism: Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Strain: HB27 / ATCC BAA-163 / DSM 7039
Molecular weight
Experimental Theoretical Method
- 109 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
DNA [2]
DNA
Name: template strand DNA
Number of copies: 1
Natural source
Organism: Thermus thermophilus
Molecular weight
Experimental Theoretical Method
- 19 kDa -
DNA
Name: nontemplate strand DNA
Number of copies: 1
Natural source
Organism: Thermus thermophilus
Molecular weight
Experimental Theoretical Method
- 19 kDa -
Ligand [3]
Name: ZINC ION
HET code: ZN
Number of copies: 2
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 65 Da -
Name: MAGNESIUM ION
HET code: MG
Number of copies: 1
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -
Name: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
HET code: AGS
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 523 Da -