EMD-2974

Single-particle
4.4 Å
EMD-2974
3D View Gallery
Deposition: 03/04/2015
Map released: 17/06/2015
Last modified: 12/08/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2974
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4uis summary report (pdf.gz) Map and 4uis FULL report (pdf.gz)
EMICSS annotations (xml)

The cryoEM map of human gamma-Secretase complex

EMD-2974

Single-particle
4.4 Å
EMD-2974
3D View Gallery
Deposition: 03/04/2015
Map released: 17/06/2015
Last modified: 12/08/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 4.2 mg/mL
Buffer
pH: 7.4
Details: 0.1% digitonin, 25 mM HEPES, pH 7.4, and 150 mM NaCl.
Grid
Details: Quantifoil Cu R1.2/1.3 grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Method: Blot for 3 seconds before plunging
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 1.4 mm
Nominal defocus: 1.5 µm - 3.0 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: DIRECT ELECTRON DE-12 (4k x 3k)
Number of real images: 3312
Average electron dose per image: 4.5 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.4 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 177207
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of T4-lysozyme fusion gamma-secretase
Details: ::::EMDATABANK.org::::EMD-2974::::
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 1.32 Å 1.32 Å 1.32 Å
Contour list
Primary Level Source
True 0.02 AUTHOR