EMD-23538 Experiments and Validation

DARPin HAS local refinement

Single particle reconstruction
Overview of EMD-23538
Sample name: DARPin D2-21.8.HSA-C9.v2
Organism: synthetic construct

Map parameters

Recommended contour level: 0.40 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.05 × 1.05 × 1.05 Å
Minimum density: -1.156
Maximum density: 3.178
Average density: 0.001
Standard deviation: 0.027

Sample information

Sample name: DARPin D2-21.8.HSA-C9.v2
Proteins: DARPin D2-21.8.HSA-C9.v2, DARPin 21.8.HSA-C9.v2

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: cryoSPARC
Number of particles: 164745

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 70.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE