EMD-23036
CryoEM structure of Yeast TFIIK (Kin28/Ccl1/Tfb3) Complex
EMD-23036
Single-particle3.5 Å
Deposition: 24/11/2020Map released: 28/04/2021
Last modified: 28/04/2021
Name: Ternary complex of Kin28-Ccl1-Tfb3 from Saccharomyces cerevisiae.
Summary:
Name: Ternary complex of Kin28-Ccl1-Tfb3 from Saccharomyces cerevisiae.
Details: The yeast Cdk7 complex, that phosphorylates the RNA pol II C-terminal domain (CTD) in transcription initiation.
Details: The yeast Cdk7 complex, that phosphorylates the RNA pol II C-terminal domain (CTD) in transcription initiation.
Natural source [1]
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| 73 kDa | - | - |
Complex Portal [1]
| ID | Name | Overlap |
|---|---|---|
| CPX-1660 | General transcription factor complex TFIIK | 1.00 |
Name: RNA polymerase II transcription factor B subunit 3
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
positive regulation of cyclin-dependent protein serine/threonine kinase activity transcription initiation at RNA polymerase II promoter transcription by RNA polymerase II nucleotide-excision repair regulation of cyclin-dependent protein serine/threonine kinase activity transcription initiation from RNA polymerase II promoter regulation of transcription by RNA polymerase II DNA repair phosphorylation of RNA polymerase II C-terminal domain
Molecular function:
cyclin-dependent protein serine/threonine kinase activator activity metal ion binding
Cellular location:
transcription factor TFIIK complex transcription factor TFIIH holo complex
positive regulation of cyclin-dependent protein serine/threonine kinase activity transcription initiation at RNA polymerase II promoter transcription by RNA polymerase II nucleotide-excision repair regulation of cyclin-dependent protein serine/threonine kinase activity transcription initiation from RNA polymerase II promoter regulation of transcription by RNA polymerase II DNA repair phosphorylation of RNA polymerase II C-terminal domain
Molecular function:
cyclin-dependent protein serine/threonine kinase activator activity metal ion binding
Cellular location:
transcription factor TFIIK complex transcription factor TFIIH holo complex
Natural source
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 38 kDa | - |
Name: Serine/threonine-protein kinase KIN28
EC number: 2.7.11.23
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
EC number: 2.7.11.23
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [29]
Biological process:
positive regulation of transcription elongation by RNA polymerase II recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex transcription initiation at RNA polymerase II promoter 7-methylguanosine mRNA capping transcription initiation from RNA polymerase II promoter positive regulation of autophagy positive regulation of Atg1/ULK1 kinase complex assembly cell division positive regulation of transcription by RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in positive regulation of transcription elongation from RNA polymerase II promoter cell cycle phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex cellular response to nitrogen starvation nucleotide-excision repair transcription by RNA polymerase I transcription by RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex phosphorylation of RNA polymerase II C-terminal domain protein phosphorylation
Molecular function:
cyclin-dependent protein serine/threonine kinase activity RNA polymerase II CTD heptapeptide repeat kinase activity ATP binding protein kinase activity
Cellular location:
cytoplasm nucleus cytosol transcription factor TFIIK complex transcription factor TFIIH holo complex
positive regulation of transcription elongation by RNA polymerase II recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex transcription initiation at RNA polymerase II promoter 7-methylguanosine mRNA capping transcription initiation from RNA polymerase II promoter positive regulation of autophagy positive regulation of Atg1/ULK1 kinase complex assembly cell division positive regulation of transcription by RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in positive regulation of transcription elongation from RNA polymerase II promoter cell cycle phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex cellular response to nitrogen starvation nucleotide-excision repair transcription by RNA polymerase I transcription by RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex phosphorylation of RNA polymerase II C-terminal domain protein phosphorylation
Molecular function:
cyclin-dependent protein serine/threonine kinase activity RNA polymerase II CTD heptapeptide repeat kinase activity ATP binding protein kinase activity
Cellular location:
cytoplasm nucleus cytosol transcription factor TFIIK complex transcription factor TFIIH holo complex
Natural source
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 35 kDa | - |
Name: Cyclin CCL1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
transcription initiation from RNA polymerase II promoter transcription initiation at RNA polymerase II promoter transcription by RNA polymerase II nucleotide-excision repair cellular response to nitrogen starvation regulation of transcription by RNA polymerase II positive regulation of Atg1/ULK1 kinase complex assembly phosphorylation of RNA polymerase II C-terminal domain regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of autophagy
Molecular function:
cyclin-dependent protein serine/threonine kinase regulator activity
Cellular location:
nucleus transcription factor TFIIH holo complex mitochondrion transcription factor TFIIK complex
transcription initiation from RNA polymerase II promoter transcription initiation at RNA polymerase II promoter transcription by RNA polymerase II nucleotide-excision repair cellular response to nitrogen starvation regulation of transcription by RNA polymerase II positive regulation of Atg1/ULK1 kinase complex assembly phosphorylation of RNA polymerase II C-terminal domain regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of autophagy
Molecular function:
cyclin-dependent protein serine/threonine kinase regulator activity
Cellular location:
nucleus transcription factor TFIIH holo complex mitochondrion transcription factor TFIIK complex
Natural source
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 45 kDa | - |
Name: ADENOSINE-5'-DIPHOSPHATE
HET code: ADP
Number of copies: 1
ChEMBL ChEBI DrugBank
HET code: ADP
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 427 Da | - |
Name: ALUMINUM FLUORIDE
HET code: AF3
Number of copies: 1
ChEBI
HET code: AF3
Number of copies: 1
ChEBI
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 83 Da | - |