EMD-22898 Experiments and Validation

Cryo-EM structure of SARS-CoV-2 ORF3a

Single particle reconstruction
Overview of EMD-22898
Sample name: SARS-CoV-2 protein 3A in lipid nanodiscs
Organisms: Severe acute respiratory syndrome coronavirus 2, Homo sapiens
Fitted atomic model: 7kjr
Related EM entries by publication: EMD-22136, EMD-22138, EMD-22139

Map parameters

Recommended contour level: 0.70 (author)
Number of grid points: 300 × 300 × 300
Voxel size: 0.73 × 0.73 × 0.73 Å
Minimum density: -2.319
Maximum density: 3.909
Average density: 0.002
Standard deviation: 0.067

Sample information

Sample name: SARS-CoV-2 protein 3A in lipid nanodiscs
Ligands: 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine, water
Proteins: SARS-CoV-2 protein 3A in lipid nanodiscs, Protein 3a, Apolipoprotein A-I

Validation information

Experimental information

 

Image processing

Number of particles: 91218

Fitting

Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.10
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100
Details: 1 blot force 5 second wait time 3 second blot time.