EMD-22492

Single-particle
3.4 Å
EMD-22492
3D View Gallery
Deposition: 20/08/2020
Map released: 14/10/2020
Last modified: 04/01/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-22492
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7jv4 summary report (pdf.gz) Map and 7jv4 FULL report (pdf.gz)
EMICSS annotations (xml)

SARS-CoV-2 spike in complex with the S2H13 neutralizing antibody (one RBD open)

EMD-22492

Single-particle
3.4 Å
EMD-22492
3D View Gallery
Deposition: 20/08/2020
Map released: 14/10/2020
Last modified: 04/01/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 70.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 137924
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
CryoSPARC - -
Startup model [1]
Type: PDB ENTRY
PDB model: 6VXX
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Sharpened map
⬡ Geometry
X Y Z
Dimensions 400 400 400
Origin 0 0 0
Spacing 400 400 400
Voxel size 1.05 Å 1.05 Å 1.05 Å
Contour list
Primary Level Source
True 0.5 AUTHOR