EMD-22473 Experiments and Validation

Cryo-EM structure of unliganded octameric prenyltransferase domain of Phomopsis amygdali fusicoccadiene synthase

Single particle reconstruction
Overview of EMD-22473
Sample name: Unliganded Phomopsis amygdali fusicoccadiene synthase octamer
Organism: Phomopsis amygdali
Fitted atomic model: 7jth

Map parameters

Recommended contour level: 0.141 (author)
Number of grid points: 384 × 384 × 384
Voxel size: 1.14 × 1.14 × 1.14 Å
Minimum density: -0.002
Maximum density: 1.855
Average density: 0.001
Standard deviation: 0.02

Sample information

Sample name: Unliganded Phomopsis amygdali fusicoccadiene synthase octamer
Proteins: Unliganded Phomopsis amygdali fusicoccadiene synthase octamer, Fusicoccadiene synthase

Validation information

Experimental information

 

Image processing

Applied symmetry: C2
Software: cryoSPARC
Number of particles: 94974
Details: Following final reconstruction, sharpening was applied using DeepEMhancer. Reconstruction before sharpening is supplied as an additional map.

Imaging

Session
Microscope model: FEI TALOS ARCTICA
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 43.0
Nominal CS (mm): 2.70
Holder model: OTHER
Acquisition
Number of images: 1500

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 2.00
Specimen support: 25 mA current was applied to grid.
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100
Details: Blot for 4.5 seconds before plunging..