EMD-22327 Experiments and Validation

Cryo-EM structure of P. falciparum VAR2CSA NF54 core in complex with CSA at 3.36 A

Single particle reconstruction
Overview of EMD-22327
Sample name: VAR2CSA NF54 core in complex with CSA
Organism: Plasmodium falciparum (isolate NF54)
Fitted atomic model: 7jgh
Related EM entries by publication: EMD-22323, EMD-22324, EMD-22325, EMD-22326

Map parameters

Recommended contour level: 3.96 (author)
Number of grid points: 300 × 300 × 300
Voxel size: 1.06 × 1.06 × 1.06 Å
Minimum density: -34.588
Maximum density: 55.299
Average density: -0.00
Standard deviation: 1.00

Sample information

Sample name: VAR2CSA NF54 core in complex with CSA
Ligand: 2-acetamido-2-deoxy-4-O-sulfo-beta-D-galactopyranose
Proteins: VAR2CSA NF54 core in complex with CSA, Erythrocyte membrane protein 1

Validation information

Experimental information

 

Image processing

Number of particles: 299571

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 57.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen support: unspecified
Vitrification
Cryogen: ETHANE