EMD-22138

Single-particle
6.5 Å
EMD-22138
3D View Gallery
Deposition: 11/06/2020
Map released: 17/06/2020
Last modified: 31/08/2022
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-22138
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Tetrameric SARS-CoV-2 ORF3a in a lipid nanodisc

EMD-22138

Single-particle
6.5 Å
EMD-22138
3D View Gallery
Deposition: 11/06/2020
Map released: 17/06/2020
Last modified: 31/08/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.7 mg/mL
Buffer
pH: 7.4
Buffer components [2]:
Name Formula Concentration ChEBI
HEPES - 20.0 mM -
Potassium chloride KCl 150.0 mM
Grid
Mesh: 300
Model: Quantifoil
Material: GOLD
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY 120.0 nm
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Details: 1 blot force, 5 second wait time, 3 second blot time.
Microscopy [1]
Microscope: FEI TALOS ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: GATAN K3 (6k x 4k)
Dimensions: 11520 pixel x 8184 pixel
Number of grids: 1
Number of real images: 7092
Average exposure time: 5.65 s
Average electron dose per image: 49.95 e/Å2
Details: 50 frames per image, 113 ms per frame
Image processing [1]
Final reconstruction
Resolution: 6.5 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 64410
Software [1]
Name Version Details
CryoSPARC 2 -
Startup model [1]
Type: OTHER
Details:Ab initio model from cryosparc 2
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2 -
Particle selection [1]
Selected Ref. model Method Software Details
1282913 - - - -
Final 3D classification
Software [1]
Name Version Details
CryoSPARC - -
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 288 288 288
Origin 0 0 0
Spacing 288 288 288
Voxel size 1.137 Å 1.137 Å 1.137 Å
Contour list
Primary Level Source
True 0.37 AUTHOR