EMD-22015 Experiments and Validation

Wt pig RyR1 in complex with apoCaM, EGTA condition (class 1 and 2, closed)

Single particle reconstruction
Overview of EMD-22015
Sample name: ryanodine receptor-FKBP1B-Calmodulin complex
Organisms: Sus scrofa, Homo sapiens, pig
Fitted atomic model: 6x32

Map parameters

Recommended contour level: 0.022 (author)
Number of grid points: 480 × 480 × 480
Voxel size: 1.09 × 1.09 × 1.09 Å
Minimum density: -0.195
Maximum density: 0.243
Average density: -0.00
Standard deviation: 0.005

Sample information

Sample name: ryanodine receptor-FKBP1B-Calmodulin complex
Ligand: ZINC ION
Proteins: ryanodine receptor-FKBP1B-Calmodulin complex, ryanodine receptor, FKBP1B, Calmodulin, Peptidyl-prolyl cis-trans isomerase FKBP1B, Ryanodine Receptor, Calmodulin-1

Validation information

Experimental information

 

Image processing

Applied symmetry: C4
Software: PHENIX
Number of particles: 44957

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen support: unspecified
Vitrification
Cryogen: ETHANE