EMD-22003
+3 extended HIV-1 reverse transcriptase initiation complex (pre-translocation state, unmasked map)
EMD-22003
Single-particle4.9 Å
Deposition: 20/05/2020Map released: 24/06/2020
Last modified: 05/08/2020
Name: +3 extended HIV-1 reverse transcriptase initiation complex core (pre-translocation state)
Summary:
Name: +3 extended HIV-1 reverse transcriptase initiation complex core (pre-translocation state)
Details: Peripheral dynamic RNA elements belonging to the tRNA lysine 3 primer and the HIV-1 viral RNA genomic template have been masked out during cryo-EM data processing.
Details: Peripheral dynamic RNA elements belonging to the tRNA lysine 3 primer and the HIV-1 viral RNA genomic template have been masked out during cryo-EM data processing.
Natural source [1]
Organism: Human immunodeficiency virus 1
Recombinant Expression [1]
| Organism | Strain | Cell | Plasmid |
|---|---|---|---|
| Escherichia coli | - | - | - |
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 25 kDa | - |
Name: HIV-1 reverse transcriptase
Details: The C-terminus of p66 contains an unstructured linker and a six-histidine tag that was cleaved prior to full complex formation.
Details: The C-terminus of p66 contains an unstructured linker and a six-histidine tag that was cleaved prior to full complex formation.
Natural source [1]
Organism: Human immunodeficiency virus 1
Recombinant Expression [1]
| Organism | Strain | Cell | Plasmid |
|---|---|---|---|
| Escherichia coli | - | - | - |
Name: HIV-1 RNA genome fragment
Details: A fragment of the HIV-1 RNA genome that is 101 nucleotides in length.
Details: A fragment of the HIV-1 RNA genome that is 101 nucleotides in length.
Natural source [1]
Organism: Human immunodeficiency virus 1
Recombinant Expression [1]
| Organism | Strain | Cell | Plasmid |
|---|---|---|---|
| Escherichia coli | - | - | - |
Name: tRNA lysine 3
Details: Chemically synthesized and extended tRNA lysine 3. A modified dG containing an N2-cystamine was placed at position 71 and the sequence was extended on the 3' end by a dC, dT, and ddG to bring its total length to 79 nucleotides.
Details: Chemically synthesized and extended tRNA lysine 3. A modified dG containing an N2-cystamine was placed at position 71 and the sequence was extended on the 3' end by a dC, dT, and ddG to bring its total length to 79 nucleotides.
Natural source [1]
Organism: Homo sapiens
Recombinant Expression [1]
| Organism | Strain | Cell | Plasmid |
|---|---|---|---|
| Escherichia coli | - | - | - |