EMD-21971

Single-particle
2.8 Å
EMD-21971
3D View Gallery
Deposition: 13/05/2020
Map released: 16/09/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21971
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6wz9 summary report (pdf.gz) Map and 6wz9 FULL report (pdf.gz)
EMICSS annotations (xml)

Bridging of double-strand DNA break activates PARP2/HPF1 to modify chromatin

EMD-21971

Single-particle
2.8 Å
EMD-21971
3D View Gallery
Deposition: 13/05/2020
Map released: 16/09/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K3 BIOQUANTUM (6k x 4k)
Detector mode: COUNTING
Average electron dose per image: 80.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 934000
⌯ Applied Symmetry
Point group: C2
Startup model [1]
Type: EMDB MAP
⦨ Initial angle assignment
Type: OTHER
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Nucleosome 1
⬡ Geometry
X Y Z
Dimensions 480 480 480
Origin 0 0 0
Spacing 480 480 480
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.04 AUTHOR