EMD-21951

Single-particle
2.0 Å
EMD-21951
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Deposition: 09/05/2020
Map released: 20/05/2020
Last modified: 06/03/2024
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EMD-21951
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Cryo-EM Structure of Human Apoferritin Light Chain Vitrified Using Back-it-up

EMD-21951

Single-particle
2.0 Å
EMD-21951
3D View Gallery
Deposition: 09/05/2020
Map released: 20/05/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Grid
Model: Homemade
Material: COPPER/RHODIUM
Details: Both sides of the grid were glow discharged for 120 seconds.
Pretreatment
Type: GLOW DISCHARGE
Time: {'units': 's', 'valueOf_': '120'}
Atmosphere: AIR
Support Film [1]
Material Topology Thickness
GOLD HOLEY ARRAY -
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 50%
Chamber temperature: 298 K
Instrument: HOMEMADE PLUNGER
Details: Back-it-up (ultrasonic specimen application and through-grid wicking in a high-speed specimen preparation device) was used.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Image Recording [1]
Detector model: FEI FALCON IV (4k x 4k)
Dimensions: 4096 pixel x 4096 pixel
Number of grids: 2
Number of real images: 3168
Average exposure time: 9.0 s
Average electron dose per image: 27.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 594259
⌯ Applied Symmetry
Point group: O
Software [1]
Name Version Details
CryoSPARC 2 -
Startup model [1]
Type: INSILICO MODEL
Insilico model: Made using cryoSPARC 2 ab initio refinement
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2 -
Particle selection [1]
Selected Ref. model Method Software Details
673794 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Sharpened map
⬡ Geometry
X Y Z
Dimensions 288 288 288
Origin 0 0 0
Spacing 288 288 288
Voxel size 0.816 Å 0.816 Å 0.816 Å
Contour list
Primary Level Source
True 1.5 AUTHOR