EMD-21868 Experiments and Validation

Cryo-EM of the S. islandicus filamentous virus, SIFV

Helical reconstruction
Overview of EMD-21868
Sample name: Sulfolobus islandicus filamentous virus
Organisms: Sulfolobus islandicus filamentous virus, SIFV
Fitted atomic model: 6wq2
Related EM entry by publication: EMD-21867

Map parameters

Recommended contour level: 0.034 (author)
Number of grid points: 320 × 320 × 320
Voxel size: 1.40 × 1.40 × 1.40 Å
Minimum density: -0.033
Maximum density: 0.085
Average density: 0.003
Standard deviation: 0.009

Sample information

Sample name: Sulfolobus islandicus filamentous virus
Nucleic acids: A-DNA, A-DNA
Proteins: Structural protein MCP2, Structural protein MCP1
Virus: Sulfolobus islandicus filamentous virus

Validation information

Experimental information

 

Image processing

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 44.0
Holder model: OTHER

Specimen

Preparation
Specimen state: filament
Specimen support: unspecified
Aggregation parameters (helical parameters)
Phi increment (degrees): 38.490000000000002
Azimuthal (Z) increment (Å): 5.4800000000000004
Rotational symmetry: C1
Vitrification
Cryogen: ETHANE