EMD-21733

Single-particle
20.0 Å
EMD-21733
3D View Gallery
Deposition: 17/04/2020
Map released: 14/10/2020
Last modified: 14/10/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21733
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Polyclonal immune complex of Fab binding the stem of H1 HA from serum of participant 4 at day 60

EMD-21733

Single-particle
20.0 Å
EMD-21733
3D View Gallery
Deposition: 17/04/2020
Map released: 14/10/2020
Last modified: 14/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Staining
Type: NEGATIVE
Material: 2% w/v uranyl formate
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: DARK FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Image Recording [1]
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Average electron dose per image: 25.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 6425
Startup model [1]
Type: PDB ENTRY
PDB model: 4K62
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Polyclonal immune complex of Fab binding the stem of H1 HA from serum of participant 4 at day 60
⬡ Geometry
X Y Z
Dimensions 160 160 160
Origin 0 0 0
Spacing 160 160 160
Voxel size 1.7700001 Å 1.7700001 Å 1.7700001 Å
Contour list
Primary Level Source
True 0.0183 AUTHOR