EMD-21714

Single-particle
20.0 Å
EMD-21714
3D View Gallery
Deposition: 16/04/2020
Map released: 14/10/2020
Last modified: 14/10/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21714
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Polyclonal immune complex of Fab binding the side of the head of H1 HA from serum of participant 11 at day 0

EMD-21714

Single-particle
20.0 Å
EMD-21714
3D View Gallery
Deposition: 16/04/2020
Map released: 14/10/2020
Last modified: 14/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Staining
Type: NEGATIVE
Material: 2% w/v uranyl formate
Microscopy [1]
Microscope: FEI TALOS ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: DARK FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 25.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1561
Startup model [1]
Type: PDB ENTRY
PDB model: 4K62
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Polyclonal immune complex of Fab binding the side of the head of H1 HA from serum of participant 11 at day 0
⬡ Geometry
X Y Z
Dimensions 160 160 160
Origin 0 0 0
Spacing 160 160 160
Voxel size 1.9799999 Å 1.9799999 Å 1.9799999 Å
Contour list
Primary Level Source
True 0.028 AUTHOR