EMD-21658
Cryo-EM structure of human Cohesin-NIPBL-DNA complex
EMD-21658
Single-particle5.3 Å
Deposition: 04/04/2020
Map released: 20/05/2020
Last modified: 06/03/2024
Name: Human Cohesin-NIPBL-DNA Complex
Summary:
Human Cohesin-NIPBL-DNA Complex
Name: Natural source [1]
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 820 kDa | - |
Complex Portal [2]
ID | Name | Overlap |
---|---|---|
CPX-5989 | Nuclear mitotic cohesin complex, STAG1 variant | 0.80 |
CPX-5989 | Nuclear meiotic cohesin complex, STAG1 variant | 0.80 |
Structural maintenance of chromosomes protein 1A
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [33]
Biological process:
establishment of mitotic sister chromatid cohesion somatic stem cell population maintenance establishment of meiotic sister chromatid cohesion response to radiation mitotic spindle assembly stem cell population maintenance sister chromatid cohesion mitotic sister chromatid cohesion DNA repair cell division response to DNA damage checkpoint signaling meiotic cell cycle mitotic sister chromatid segregation
Molecular function:
chromatin binding protein heterodimerization activity ATP binding DNA binding RNA binding mediator complex binding ATP hydrolysis activity
Cellular location:
cytosol mitotic cohesin complex cohesin complex nuclear meiotic cohesin complex nucleus nucleoplasm mitotic spindle pole chromosome, centromeric region nuclear matrix condensed nuclear chromosome kinetochore meiotic cohesin complex chromosome
establishment of mitotic sister chromatid cohesion somatic stem cell population maintenance establishment of meiotic sister chromatid cohesion response to radiation mitotic spindle assembly stem cell population maintenance sister chromatid cohesion mitotic sister chromatid cohesion DNA repair cell division response to DNA damage checkpoint signaling meiotic cell cycle mitotic sister chromatid segregation
Molecular function:
chromatin binding protein heterodimerization activity ATP binding DNA binding RNA binding mediator complex binding ATP hydrolysis activity
Cellular location:
cytosol mitotic cohesin complex cohesin complex nuclear meiotic cohesin complex nucleus nucleoplasm mitotic spindle pole chromosome, centromeric region nuclear matrix condensed nuclear chromosome kinetochore meiotic cohesin complex chromosome
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 143 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Trichoplusia ni | - | - | - |
Structural maintenance of chromosomes protein 3
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [36]
Biological process:
establishment of mitotic sister chromatid cohesion establishment of meiotic sister chromatid cohesion mitotic sister chromatid cohesion sister chromatid cohesion meiotic cell cycle mitotic cell cycle mitotic spindle assembly DNA repair regulation of DNA replication cell division stem cell population maintenance
Molecular function:
cohesin loader activity double-stranded DNA binding chromatin binding microtubule motor activity protein heterodimerization activity mediator complex binding cohesin loading activity beta-tubulin binding ATP hydrolysis activity dynein complex binding ATP binding
Cellular location:
mitotic cohesin complex nucleus nuclear meiotic cohesin complex nuclear matrix cohesin complex cytosol nucleoplasm meiotic cohesin complex mitotic spindle pole lateral element nuclear mitotic cohesin complex chromosome chromosome, centromeric region chromatin
establishment of mitotic sister chromatid cohesion establishment of meiotic sister chromatid cohesion mitotic sister chromatid cohesion sister chromatid cohesion meiotic cell cycle mitotic cell cycle mitotic spindle assembly DNA repair regulation of DNA replication cell division stem cell population maintenance
Molecular function:
cohesin loader activity double-stranded DNA binding chromatin binding microtubule motor activity protein heterodimerization activity mediator complex binding cohesin loading activity beta-tubulin binding ATP hydrolysis activity dynein complex binding ATP binding
Cellular location:
mitotic cohesin complex nucleus nuclear meiotic cohesin complex nuclear matrix cohesin complex cytosol nucleoplasm meiotic cohesin complex mitotic spindle pole lateral element nuclear mitotic cohesin complex chromosome chromosome, centromeric region chromatin
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 141 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Trichoplusia ni | - | - | - |
Double-strand-break repair protein rad21 homolog
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [45]
Biological process:
positive regulation of interleukin-10 production negative regulation of interleukin-1 beta production negative regulation of neuron apoptotic process response to hypoxia negative regulation of tumor necrosis factor production negative regulation of glial cell apoptotic process chromosome segregation establishment of mitotic sister chromatid cohesion chromatin looping sister chromatid cohesion establishment of meiotic sister chromatid cohesion reciprocal meiotic recombination double-strand break repair positive regulation of sister chromatid cohesion mitotic sister chromatid cohesion apoptotic process regulation of transcription by RNA polymerase II negative regulation of G2/M transition of mitotic cell cycle negative regulation of mitotic metaphase/anaphase transition protein localization to chromatin DNA recombination replication-born double-strand break repair via sister chromatid exchange synaptonemal complex assembly cell division
Molecular function:
lncRNA binding DNA-binding transcription factor binding DNA binding chromatin binding
Cellular location:
meiotic cohesin complex mitotic cohesin complex condensed nuclear chromosome nucleus midbody chromosome, centromeric region spindle pole nuclear matrix chromosome membrane cohesin complex nuclear meiotic cohesin complex nuclear mitotic cohesin complex chromatin synaptonemal complex nucleoplasm cytosol
positive regulation of interleukin-10 production negative regulation of interleukin-1 beta production negative regulation of neuron apoptotic process response to hypoxia negative regulation of tumor necrosis factor production negative regulation of glial cell apoptotic process chromosome segregation establishment of mitotic sister chromatid cohesion chromatin looping sister chromatid cohesion establishment of meiotic sister chromatid cohesion reciprocal meiotic recombination double-strand break repair positive regulation of sister chromatid cohesion mitotic sister chromatid cohesion apoptotic process regulation of transcription by RNA polymerase II negative regulation of G2/M transition of mitotic cell cycle negative regulation of mitotic metaphase/anaphase transition protein localization to chromatin DNA recombination replication-born double-strand break repair via sister chromatid exchange synaptonemal complex assembly cell division
Molecular function:
lncRNA binding DNA-binding transcription factor binding DNA binding chromatin binding
Cellular location:
meiotic cohesin complex mitotic cohesin complex condensed nuclear chromosome nucleus midbody chromosome, centromeric region spindle pole nuclear matrix chromosome membrane cohesin complex nuclear meiotic cohesin complex nuclear mitotic cohesin complex chromatin synaptonemal complex nucleoplasm cytosol
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 71 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Trichoplusia ni | - | - | - |
Cohesin subunit SA-1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [18]
Biological process:
establishment of mitotic sister chromatid cohesion localization establishment of meiotic sister chromatid cohesion sister chromatid cohesion mitotic spindle assembly cell division
Molecular function:
chromatin binding
Cellular location:
mitotic cohesin complex nucleus chromosome, centromeric region mitotic spindle pole chromosome cytosol nuclear body nuclear matrix chromatin cohesin complex nucleoplasm
establishment of mitotic sister chromatid cohesion localization establishment of meiotic sister chromatid cohesion sister chromatid cohesion mitotic spindle assembly cell division
Molecular function:
chromatin binding
Cellular location:
mitotic cohesin complex nucleus chromosome, centromeric region mitotic spindle pole chromosome cytosol nuclear body nuclear matrix chromatin cohesin complex nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 146 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Trichoplusia ni | - | - | - |
Nipped-B-like protein
Number of copies: 1
UniProtKB UniProtKB PDBe-KB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB
Domains
Gene Ontology [2]
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 188 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Trichoplusia ni | - | - | - |
DNA (51-MER)
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 15 kDa | - |
DNA (51-MER)
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 15 kDa | - |
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
HET code: ANP
Number of copies: 2
ChEMBL ChEBI
Name: HET code: ANP
Number of copies: 2
ChEMBL ChEBI
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 506 Da | - |