EMD-21632

Single-particle
3.2 Å
EMD-21632
3D View Gallery
Deposition: 31/03/2020
Map released: 01/07/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21632
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6wdd summary report (pdf.gz) Map and 6wdd FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure V-A)

EMD-21632

Single-particle
3.2 Å
EMD-21632
3D View Gallery
Deposition: 31/03/2020
Map released: 01/07/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Frames per image: 1-35
Number of grids: 1
Number of real images: 3218
Average exposure time: 1.0 s
Average electron dose per image: 35.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 32253
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN - -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-1003
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
FREALIGN - -
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
FREALIGN - -
Particle selection [1]
Selected Ref. model Method Software Details
678268 - - - -
Final 3D classification
Software [1]
Name Version Details
FREALIGN - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Map V-A resolution filtered with blocfilt with B-factor of -50 A2 applied
⬡ Geometry
X Y Z
Dimensions 288 288 288
Origin 0 0 0
Spacing 288 288 288
Voxel size 1.333 Å 1.333 Å 1.333 Å
Contour list
Primary Level Source
True 1.0 AUTHOR