EMD-21518 Experiments and Validation

Cryo-EM structure of 5HT3A receptor in presence of Palonosetron

Single particle reconstruction
Overview of EMD-21518
Sample name: Serotonin receptor
Organism: Mus musculus
Fitted atomic model: 6w1y
Related EM entry by publication: EMD-21511, EMD-21512

Map parameters

Recommended contour level: 0.008 (author)
Number of grid points: 300 × 300 × 300
Voxel size: 0.85 × 0.85 × 0.85 Å
Minimum density: -0.024
Maximum density: 0.048
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: Serotonin receptor
Ligand: (3~{a}~{S})-2-[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]-3~{a},4,5,6-tetrahydro-3~{H}-benzo[de]isoquinolin-1-one
Proteins: Serotonin receptor, 5-hydroxytryptamine receptor 3A

Validation information

Experimental information

 

Image processing

Software: RELION
Number of particles: 91163

Fitting

Overall B value: 70.0
Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Nominal CS (mm): 2.70
Nominal magnification: 105000.0
Holder model: OTHER
Acquisition
Number of images: 5970

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 3.00
Vitrification
Apparatus: FEI VITROBOT MARK I
Cryogen: ETHANE
Humidity: 100
Details: blot for 2.5 seconds.