EMD-21499

Single-particle
22.0 Å
EMD-21499
3D View Gallery
Deposition: 01/03/2020
Map released: 01/07/2020
Last modified: 01/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21499
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

BG505 SOSIP.664 in complex with rabbit Fab 43A1

EMD-21499

Single-particle
22.0 Å
EMD-21499
3D View Gallery
Deposition: 01/03/2020
Map released: 01/07/2020
Last modified: 01/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Staining
Type: NEGATIVE
Material: Uranyl Formate
Microscopy [1]
Microscope: FEI TALOS ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Image Recording [1]
Detector model: FEI CETA (4k x 4k)
Average electron dose per image: 25.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 22.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 3483
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
RELION - -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: BG505 SOSIP.664 in complex with rabbit Fab 43A1
⬡ Geometry
X Y Z
Dimensions 192 192 192
Origin 0 0 0
Spacing 192 192 192
Voxel size 1.98 Å 1.98 Å 1.98 Å
Contour list
Primary Level Source
True 0.014 AUTHOR