EMD-21413

Subtomogram averaging
9.9 Å
EMD-21413 Deposition: 19/02/2020
Map released: 01/07/2020
Last modified: 19/08/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21413

HIV envelope glycoprotein bound with antibodies 10-1074 and 3BNC117 on AT-2 treated BaL strain virus

EMD-21413

Subtomogram averaging
9.9 Å
EMD-21413 Deposition: 19/02/2020
Map released: 01/07/2020
Last modified: 19/08/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: DARK FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 9.9 Å ( BY AUTHOR)
Resolution method: OTHER
Applied Symmetry
Point group: C3
Final angle assignment
Type: OTHER
Extraction
Number of images used: 16937
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: HIV envelope glycoprotein bound with antibodies 10-1074 and 3BNC117 on AT-2 treated BaL strain virus
Geometry
X Y Z
Dimensions 128 128 128
Origin -64 -64 -64
Spacing 128 128 128
Voxel size 2.2 Å 2.2 Å 2.2 Å
Contour list
Primary Level Source
True 0.122 AUTHOR