EMD-21218
Cryo-EM reconstruction of incomplete 80S ribosome (class v)
EMD-21218
Single-particle9.8 Å
Deposition: 16/01/2020
Map released: 14/10/2020
Last modified: 20/01/2021
Buffer
pH: 8.0
Grid
Vitrification
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Average exposure time: 8.0 s
Average electron dose per image: 23.0 e/Å2
Average exposure time: 8.0 s
Average electron dose per image: 23.0 e/Å2
Final
reconstruction
Resolution: 9.8
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 7081
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 7081
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.2 | - |
⦨ Initial angle
assignment
⦩ Final angle assignment
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of incomplete 80S ribosome (class v)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of incomplete 80S ribosome (class v)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 276 | 276 | 276 |
Origin | 0 | 0 | 0 |
Spacing | 276 | 276 | 276 |
Voxel size | 1.66 Å | 1.66 Å | 1.66 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.015 | AUTHOR |