EMD-21218

Single-particle
9.8 Å
EMD-21218
3D View Gallery
Deposition: 16/01/2020
Map released: 14/10/2020
Last modified: 20/01/2021
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21218
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Cryo-EM reconstruction of incomplete 80S ribosome (class v)

EMD-21218

Single-particle
9.8 Å
EMD-21218
3D View Gallery
Deposition: 16/01/2020
Map released: 14/10/2020
Last modified: 20/01/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Grid
Model: Quantifoil R2/4
Material: COPPER
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average exposure time: 8.0 s
Average electron dose per image: 23.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 9.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 7081
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 1.2 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 1.2 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 1.2 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of incomplete 80S ribosome (class v)
⬡ Geometry
X Y Z
Dimensions 276 276 276
Origin 0 0 0
Spacing 276 276 276
Voxel size 1.66 Å 1.66 Å 1.66 Å
Contour list
Primary Level Source
True 0.015 AUTHOR