EMD-21035

Single-particle
3.7 Å
EMD-21035
3D View Gallery
Deposition: 25/11/2019
Map released: 01/07/2020
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21035
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6v3f summary report (pdf.gz) Map and 6v3f FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of NPC1-like intracellular cholesterol transporter 1 (NPC1L1)

EMD-21035

Single-particle
3.7 Å
EMD-21035
3D View Gallery
Deposition: 25/11/2019
Map released: 01/07/2020
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 5 mg/mL
Buffer
pH: 7.5
Grid
Mesh: 300
Model: Quantifoil R1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K3 BIOQUANTUM (6k x 4k)
Average electron dose per image: 46.76 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 144908
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
CTF correction
Software [1]
Name Version Details
Gctf 1.06 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of full-length NPC1L1
⬡ Geometry
X Y Z
Dimensions 320 320 320
Origin 0 0 0
Spacing 320 320 320
Voxel size 0.862 Å 0.862 Å 0.862 Å
Contour list
Primary Level Source
True 0.011 AUTHOR