EMD-20725

Single-particle
4.48 Å
EMD-20725 Deposition: 13/09/2019
Map released: 25/03/2020
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20725

Human IMPDH2 treated with ATP and 2 mM GTP. Free canonical octamer reconstruction.

EMD-20725

Single-particle
4.48 Å
EMD-20725 Deposition: 13/09/2019
Map released: 25/03/2020
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 100.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.48 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 26847
Applied Symmetry
Point group: D4
Startup model [1]
Type: EMDB MAP
Initial angle assignment
Final angle assignment
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human IMPDH2 treated with ATP and 2 mM GTP. Free canonical octamer reconstruction.
Geometry
X Y Z
Dimensions 256 256 256
Origin 0 0 0
Spacing 256 256 256
Voxel size 1.05 Å 1.05 Å 1.05 Å
Contour list
Primary Level Source
True 0.02 AUTHOR