EMD-20725
Human IMPDH2 treated with ATP and 2 mM GTP. Free canonical octamer reconstruction.
EMD-20725
Single-particle4.48 Å
Deposition: 13/09/2019
Map released: 25/03/2020
Last modified: 20/03/2024
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Final
reconstruction
Resolution: 4.48
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 26847
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 26847
⌯ Applied Symmetry
Point group:
D4
Startup model
[1]
Type:
EMDB MAP
⦨ Initial angle
assignment
Type:
MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type:
MAXIMUM LIKELIHOOD
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human IMPDH2 treated with ATP and 2 mM GTP. Free canonical octamer reconstruction.
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human IMPDH2 treated with ATP and 2 mM GTP. Free canonical octamer reconstruction.
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 256 | 256 | 256 |
Origin | 0 | 0 | 0 |
Spacing | 256 | 256 | 256 |
Voxel size | 1.05 Å | 1.05 Å | 1.05 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.02 | AUTHOR |