Electron Microscopy Data Bank

EMD-20674

Subtomogram averaging
35.6 Å

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Deposition: 04/09/2019
Map released: 06/11/2019
Last modified: 06/09/2023

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EMD-20674

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Nascent axoneme from bovine respiratory epithelial cells

EMD-20674

Subtomogram averaging
35.6 Å
EMD-20674

3D View Gallery

Deposition: 04/09/2019
Map released: 06/11/2019
Last modified: 06/09/2023

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Subtomogram averaging
Aggregation State: Filament

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Buffer

pH: 7.4

Grid

Mesh: 200
Model: Quantifoil R2/2
Material: COPPER/RHODIUM

Pretreatment

Type: GLOW DISCHARGE
Time: {'units': 's', 'valueOf_': '25'}
Atmosphere:

Vitrification

Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: FEI VITROBOT MARK IV
Details: 15 second blot.

Microscopy [1]

Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 100.0 µm

Image Recording [1]

Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 0.8 e/Å²

Image processing [1]

Final reconstruction

Resolution: 35.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Algorithm: BACK PROJECTION

Applied Symmetry

Point group: C1

Final angle assignment

Type: MAXIMUM LIKELIHOOD

Extraction

Number of images used: 500

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Proximal axoneme from bovine respiratory epithelium

Geometry

	X	Y	Z
Dimensions	200	200	200
Origin	0	0	0
Spacing	200	200	200
Voxel size	8.18 Å	8.18 Å	8.18 Å

Contour list

Primary	Level	Source
True	0.3	AUTHOR