EMD-20668

Single-particle
3.1 Å
EMD-20668
3D View Gallery
Deposition: 02/09/2019
Map released: 13/11/2019
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20668
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6u7h summary report (pdf.gz) Map and 6u7h FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the HCoV-229E spike glycoprotein

EMD-20668

Single-particle
3.1 Å
EMD-20668
3D View Gallery
Deposition: 02/09/2019
Map released: 13/11/2019
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.8 mg/mL
Details: This sample was monodisperse.
Buffer
pH: 8.0
Buffer components [2]:
Name Formula Concentration ChEBI
Sodium chloride NaCl 150.0 mM
Tris C4H11NO3 10.0 mM
Grid
Mesh: 400
Material: COPPER
Details: 15 micro Ampere
Pretreatment
Type: GLOW DISCHARGE
Atmosphere: AIR
Pressure: 0.04 kPa
Support Film [1]
Material Topology Thickness
- HOLEY ARRAY 3.5 nm
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: GATAN CRYOPLUNGE 3
Details: Blot for 13 seconds before plunging.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal magnification: 75000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Dimensions: 4096 pixel x 4096 pixel
Average exposure time: 60.0 s
Average electron dose per image: 42.7 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 71350
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
CryoSPARC 2.90 -
Startup model [1]
Type: NONE
Details:A initial 3D map was generated by ab-initio reconstruction in cryoSPARC v2.
⦨ Initial angle assignment
Type: OTHER
⦩ Final angle assignment
Type: OTHER
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1.10 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 280 280 280
Origin 0 0 0
Spacing 280 280 280
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.26 AUTHOR