EMD-20668
Cryo-EM structure of the HCoV-229E spike glycoprotein
EMD-20668
Single-particle3.1 Å
Deposition: 02/09/2019
Map released: 13/11/2019
Last modified: 02/12/2020
Concentration: 0.8
mg/mL
Details: This sample was monodisperse.
Details: This sample was monodisperse.
Buffer
pH: 8.0
Buffer components [2]:
Buffer components [2]:
Name | Formula | Concentration | ChEBI |
---|---|---|---|
Sodium chloride | NaCl | 150.0 mM | |
Tris | C4H11NO3 | 10.0 mM |
Grid
Mesh: 400
Material: COPPER
Details: 15 micro Ampere
Material: COPPER
Details: 15 micro Ampere
Pretreatment
Support Film [1]
Material | Topology | Thickness |
---|---|---|
- | HOLEY ARRAY | 3.5 nm |
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: GATAN CRYOPLUNGE 3
Details: Blot for 13 seconds before plunging.
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: GATAN CRYOPLUNGE 3
Details: Blot for 13 seconds before plunging.
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal magnification: 75000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal magnification: 75000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Dimensions: 4096 pixel x 4096 pixel
Average exposure time: 60.0 s
Average electron dose per image: 42.7 e/Å2
Detector mode: COUNTING
Dimensions: 4096 pixel x 4096 pixel
Average exposure time: 60.0 s
Average electron dose per image: 42.7 e/Å2
Final
reconstruction
Resolution: 3.1
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 71350
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 71350
⌯ Applied Symmetry
Point group:
C3
Software
[1]
Name | Version | Details |
---|---|---|
CryoSPARC | 2.90 | - |
Startup model
[1]
⦨ Initial angle
assignment
Type:
OTHER
⦩ Final angle assignment
Type:
OTHER
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 4.1.10 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 280 | 280 | 280 |
Origin | 0 | 0 | 0 |
Spacing | 280 | 280 | 280 |
Voxel size | 1.06 Å | 1.06 Å | 1.06 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.26 | AUTHOR |