EMD-20638 Experiments and Validation

E. coli 50S with phazolicin (PHZ) bound in exit tunnel

Single particle reconstruction
Overview of EMD-20638
Sample name: E. coli ribosome with phazolicin in exit tunnel
Organisms: Escherichia coli, Rhizobium sp. Pop5
Fitted atomic model: 6u48

Map parameters

Recommended contour level: 0.027 (author)
Number of grid points: 320 × 320 × 320
Voxel size: 1.11 × 1.11 × 1.11 Å
Minimum density: -0.141
Maximum density: 0.214
Average density: -0.00
Standard deviation: 0.008

Sample information

Sample name: E. coli ribosome with phazolicin in exit tunnel
Nucleic acids: 23S rRNA, 5S rRNA
Proteins: E. coli ribosome with phazolicin in exit tunnel, E. coli 50S, Phazolicin, 50S ribosomal protein uL2, 50S ribosomal protein uL3, 50S ribosomal protein uL4, 50S ribosomal protein uL5, 50S ribosomal protein uL6, 50S ribosomal protein bL9, 50S ribosomal protein uL11, 50S ribosomal protein uL13, 50S ribosomal protein uL14, 50S ribosomal protein uL15, 50S ribosomal protein uL16, 50S ribosomal protein bL17, 50S ribosomal protein uL18, 50S ribosomal protein bL19, 50S ribosomal protein bL20, 50S ribosomal protein bL21, 50S ribosomal protein uL22, 50S ribosomal protein uL23, 50S ribosomal protein uL24, 50S ribosomal protein bL25, 50S ribosomal protein bL27, 50S ribosomal protein bL28, 50S ribosomal protein uL29, 50S ribosomal protein uL30, 50S ribosomal protein bL32, 50S ribosomal protein bL33, 50S ribosomal protein bL34, 50S ribosomal protein bL35, 50S ribosomal protein bL36, phazolicin

Validation information

Experimental information

 

Image processing

Software: RELION
Number of particles: 65393

Fitting

Initial atomic model: 4YBB

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 30.0
Nominal CS (mm): 2.70
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen support: unspecified
Vitrification
Cryogen: ETHANE