EMD-20541

Single-particle
3.6 Å
EMD-20541
3D View Gallery
Deposition: 01/08/2019
Map released: 04/12/2019
Last modified: 05/04/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20541
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6pzz summary report (pdf.gz) Map and 6pzz FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM map of NA-80 Fab in complex with N9 Shangahi2

EMD-20541

Single-particle
3.6 Å
EMD-20541
3D View Gallery
Deposition: 01/08/2019
Map released: 04/12/2019
Last modified: 05/04/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.88 mg/mL
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal defocus: 0.8 µm - 2.0 µm
Nominal magnification: 29000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 11240
⌯ Applied Symmetry
Point group: C4
Software [1]
Name Version Details
CryoSPARC 1 -
⦨ Initial angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
CryoSPARC 1 -
⦩ Final angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
CryoSPARC 1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: CryoEM map of NA-80 Fab in complex with N9 Shanghai
⬡ Geometry
X Y Z
Dimensions 288 288 288
Origin 0 0 0
Spacing 288 288 288
Voxel size 1.03 Å 1.03 Å 1.03 Å
Contour list
Primary Level Source
True 0.641 AUTHOR