EMD-20536 Experiments and Validation

Cryo-EM Structure of the Respiratory Syncytial Virus Polymerase (L) Protein Bound by the Tetrameric Phosphoprotein (P)

Single particle reconstruction
Overview of EMD-20536
Sample name: Respiratory Syncytial Virus Polymerase (L) Protein Bound by the Tetrameric Phosphoprotein (P)
Organism: Human respiratory syncytial virus A2
Fitted atomic model: 6pzk

Map parameters

Recommended contour level: 0.35 (author)
Number of grid points: 256 × 256 × 256
Voxel size: 1.07 × 1.07 × 1.07 Å
Minimum density: -0.602
Maximum density: 1.462
Average density: 0.00
Standard deviation: 0.041

Sample information

Sample name: Respiratory Syncytial Virus Polymerase (L) Protein Bound by the Tetrameric Phosphoprotein (P)
Proteins: Respiratory Syncytial Virus Polymerase (L) Protein Bound by the Tetrameric Phosphoprotein (P), RNA-directed RNA polymerase L, Phosphoprotein

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: cryoSPARC
Number of particles: 196720

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 48.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.29
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE