EMD-20533

Single-particle
4.55 Å
EMD-20533
3D View Gallery
Deposition: 31/07/2019
Map released: 14/08/2019
Last modified: 07/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20533
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6pzb summary report (pdf.gz) Map and 6pzb FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the pancreatic beta-cell SUR1 Apo state

EMD-20533

Single-particle
4.55 Å
EMD-20533
3D View Gallery
Deposition: 31/07/2019
Map released: 14/08/2019
Last modified: 07/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.55 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 90058
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-7073
⦨ Initial angle assignment
Type: COMMON LINE
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: SUR1-Apo state
⬡ Geometry
X Y Z
Dimensions 110 110 110
Origin 20 41 30
Spacing 110 110 110
Voxel size 1.826 Å 1.826 Å 1.826 Å
Contour list
Primary Level Source
True 0.0328 AUTHOR