EMD-20532 Experiments and Validation

Toll receptor EM map from Topaz picks

Single particle reconstruction
Overview of EMD-20532
Sample name: Toll receptor
Organism: Drosophila melanogaster

Map parameters

Recommended contour level: 0.53 (author)
Number of grid points: 320 × 320 × 320
Voxel size: 0.83 × 0.83 × 0.83 Å
Minimum density: 0.00
Maximum density: 1.00
Average density: 0.011
Standard deviation: 0.098

Sample information

Sample name: Toll receptor
Protein: Toll receptor

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: cryoSPARC
Number of particles: 1006089
CTF correction: Estimated with CTFFind4, applied with cryosparc 2.9

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 73.480000000000004
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.40
Vitrification
Cryogen: ETHANE