EMD-20490 Experiments and Validation

Human alpha3beta4 nicotinic acetylcholine receptor in complex with AT-1001 in saposin nanodiscs

Single particle reconstruction
Overview of EMD-20490
Sample name: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to AT-1001
Organism: Homo sapiens
Related EM entries by publication: EMD-20487, EMD-20488, EMD-20489

Map parameters

Recommended contour level: 0.009 (author)
Number of grid points: 320 × 320 × 320
Voxel size: 0.84 × 0.84 × 0.84 Å
Minimum density: -0.02
Maximum density: 0.032
Average density: 0.00
Standard deviation: 0.002

Sample information

Sample name: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to AT-1001
Protein: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to AT-1001

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 40686
Reconstruction protocol: BACK PROJECTION

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 48.0
Energy filter: GIF Quantum LS
Nominal CS (mm): 2.70
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Acquisition
Number of images: 3993

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 6.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100
Details: 4 second blot.